We successfully overcome the frequent challenge of low quality source materials for RNA analyses by using the Illumina TruSeq RNA Access Library Prep Kit. It is ideal for formalin-fixed, paraffin-embedded (FFPE) samples as well as degraded total RNA and samples with limited starting material. While traditional poly-A based methods for capturing the coding transcriptome are ineffective for FFPE RNA-seq analysis, TruSeq RNA Access Library Prep Kits overcome this limitation by applying sequence-specific capture that does not rely on the presence of polyadenylated transcripts.
Starting from as little as 10 ng of intact total RNA and 20 ng of degraded (DV200 >35%) total RNA the Illumina TruSeq RNA Access Library Prep Kit is ideal for accurate, sensitive RNA-seq results that focus on the RNA coding regions.
- Focused Capturing for Maximum Affordability
Sequence-specific capturing maximizes discovery power at a fraction of the sequencing depth
- High-Quality Data from Difficult Samples
Optimized for sequencing RNA from degraded samples, including FFPE samples
- Ideal for Samples with Limited Starting Material
Greatly reduced sample input requirements (as little as 10 ng total RNA) while maintaining high sensitivity