We have successfully overcome the challenge of low quantity source materials for RNA analyses by using the SMARTer® Ultra™ Low Input RNA Kit, sold and supported by Clontech. It is a cDNA synthesis kit designed for single-cell or ultra-low input RNA-Seq. The kit enables robust transcriptome analysis down to single-cell input levels in high quality samples, and is optimized for Illumina sequencing.
The assay accurately synthesizes full-length cDNA from only 1-1000 whole cells or 10 pg to 10 ng high-quality total RNA. The single-tube protocol works directly on whole cells and preserves sample integrity